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| Status |
Public on Jul 18, 2017 |
| Title |
microRNA expression profiling in human colon cancer |
| Platform organisms |
Homo sapiens; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Macacine alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Herpesvirus saimiri (strain 11); JC polyomavirus; Human immunodeficiency virus 1; Human betaherpesvirus 6B; Human gammaherpesvirus 8; Merkel cell polyomavirus; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Using the highly sensitive miRCURY LNA™ microRNA array, we screened 3100 microRNAs abundant in the human colon cancer and Adjacent normal gastric mucosa tissues, and the function of differentially expressed microRNAs were analyzed by bioinformatics. The enrichment results indicated that these microRNAs perhaps participated in the occurrence and development process of colon cancer.
In this study, three cases of colon cancer were used to acquire the microRNA expression profiling, and qPCR was employed to confirm the results of microRNA chip. The function of the differentially expressed microRNA were analyzed by bioinformatic methods.Finally,compared with the adjacent normal mucosa tissues，the expression of 2 microRNAs were up regulated in colon cancer. qPCR results showed an expression pattern consistent with that of the chip analysis.
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| Overall design |
In this study, three cases of colon cancer were used to acquire the microRNA expression profiling, and qPCR was employed to confirm the results of microRNA chip. The function of the differentially expressed microRNA were analyzed by bioinformatic methods.Finally,compared with the adjacent normal mucosa tissues，the expression of 2 microRNAs were up regulated in colon cancer. qPCR results showed an expression pattern consistent with that of the chip analysis.
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| Contributor(s) |
Huang Y, Xie N |
| Citation(s) |
34109430 |
| Submission date |
Jul 17, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Yousheng Huang |
| E-mail(s) |
hys768811@163.com
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| Organization name |
Hainan Medical university
|
| Department |
Department of pathology
|
| Street address |
The 3rd Xueyuan Road
|
| City |
Haikou |
| State/province |
Hainan |
| ZIP/Postal code |
570102 |
| Country |
China |
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| Platforms (1) |
| GPL21439 |
miRCURY LNA microRNA Array, 7th generation - hsa, mmu & rno (miRBase 21; probeID version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA394696 |
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