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| Status |
Public on Nov 22, 2018 |
| Title |
Transcriptome of U251 cells overexpression complement component 7 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We identified a rare coding variant (p.K420Q) in the complement component 7 (C7) gene affecting the risk of Alzheimer's disease. To investigate the cellular effects of the mutant, we performed RNA-seq in cell line overexpression wilt-type and mutant C7. U251 glioma cells with stable expression of mutant APP (K670N/M671L) (U251-APP cells), which produce Aβ42 under Dox inducing, were used as the model cell. Total RNA of U251-APP cells overexpressing wild type and mutant C7 proteins were subjected to transcriptome sequencing using Illumina Hiseq 4000 platform.
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| |
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| Overall design |
C7 overexpression was performed in U251 cell with a stably expression of mutant APP (U251-APP cells) with three biological triplicates for each condition (vector, wild-type, and mutant).
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| |
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| Contributor(s) |
Zhang D, Fan Y, Xu M, Yao Y |
| Citation(s) |
31032141 |
| Submission date |
Jul 19, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Yong-Gang Yao |
| E-mail(s) |
yaoyg@mail.kiz.ac.cn
|
| Organization name |
Kunming Institute of Zoology
|
| Department |
Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province
|
| Street address |
32 Jiaochang Donglu
|
| City |
Kunming |
| State/province |
Yunnan |
| ZIP/Postal code |
650223 |
| Country |
China |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA394980 |
| SRA |
SRP112852 |
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