|
| Status |
Public on Oct 25, 2017 |
| Title |
Fibroblast-specific genetic dissection of TGFβ-Smad2/3 signaling in cardiac fibrosis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Overall goal: To elucidate fibroblast-specific role of TGFβ-Smad2/3 signaling in fibroblast activation and their differentiation to myofibroblasts. Purpose of analysis: To generate transcriptional profile of Smad2/3 and TGFβreceptors1/2-deficient fibroblasts in the context of pathological cardiac fibrosis.
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| |
|
| Overall design |
Experimental structure: Transcriptional profile generated using RNAseq and differential gene expression analyses of fibroblasts lacking either Smad2/3 or TGFbreceptors1/2 fibroblasts isolated from stressed/diseased hearts.
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| |
|
| Contributor(s) |
Molkentin J, Khalil H, Prasad V |
| Citation(s) |
28891814 |
| Submission date |
Jul 22, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Vikram Prasad |
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Molecular Cardiovascular Biology
|
| Street address |
240, Albert Sabin Way
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (8)
|
| GSM2715304 |
Control cells for Smad2/3 deficient fibroblasts (HK3_run1739) |
| GSM2715305 |
Control cells for Smad2/3 deficient fibroblasts (HK4_run1739) |
| GSM2715306 |
Mutant Smad2/3 DKO fibroblasts (HK15_run1739) |
| GSM2715307 |
Mutant Smad2/3 DKO fibroblasts (HK16_run1739) |
| GSM2715308 |
Control cells for Tgfb receptors1/2 -deficient fibroblasts (HK6_run2110) |
| GSM2715309 |
Control cells for Tgfb receptors1/2 -deficient fibroblasts (HK7_run2110) |
| GSM2715310 |
Mutant TGFbreceptors1/2 deficient fibroblasts (HK2_run2110) |
| GSM2715311 |
Mutant TGFbreceptors1/2 deficient fibroblasts (HK4_run2110) |
|
| Relations |
| BioProject |
PRJNA395421 |
| SRA |
SRP113371 |
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