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| Status |
Public on Oct 15, 2018 |
| Title |
Gene expression analysis of P3 Dot1l conditional knockout mice in the cerebellum and of cerebellar granular neuron progenitors (CGNPs) or cerebellar granular neurons (CGNs) isolated from P7 wt mice upon DOT1L inhibition |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
DOT1L as methyltransferase of H3K79 is implicated in brian development. Here, we further defined DOT1L function in gene expression during cerebellar development using Microarrays. For that we generated Dot1l knockout mice using a Atoh-Cre driver line resulting in a Dot1l knockout within the cerebellum. The RNA of cerebellar tissue of the Dot1l knockout animals was thereby compared to controls. Additionally we compared the RNA levels of cultured CGNP and CGN samples treated with a DOT1L inhibitor versus DMSO treated cells. The data sets reveals potential new gene expression targets of DOT1L in vivo and in vitro, which ensure a correct development of the cerebellum.
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| Overall design |
Microarray analysis using Affymetrix (which array?) arrays of the cerebellum of Dot1l conditional knockout mice versus controls. Additionally, CGNPs and CGNs were isolated at P7 of wt mice, treated with the DOT1L inhibitor SGC0946 and than analyzed via Affymetrix Microarrys.
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| Contributor(s) |
Bovio P, Pfeifer D, Bertemes H, Vogel T |
| Citation(s) |
30302725 |
| Submission date |
Jul 27, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Patrick Piero Bovio |
| Organization name |
Albert-Ludwigs University Freiburg, Institute of Anatomy and Cell Biology
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| Department |
Department of Molecular Embryology
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| Lab |
Vogel
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| Street address |
Albertstr. 19
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| City |
Freiburg |
| ZIP/Postal code |
79104 |
| Country |
Germany |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE101949 |
Cerebellar granular neurons (CGN) and progenitors (CGNP) upon DOT1L inhibition or cKO |
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| Relations |
| BioProject |
PRJNA395989 |
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