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| Status |
Public on Oct 31, 2017 |
| Title |
Characteristics and localization of prospective fibroblast subpopulations isolated from the mouse lung |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
By fluorescence-activated cell sorting, we classified lung fibroblasts highly expressing Pdgfra into three subpopulations, Sca-1highThy-1low (A-type), Sca-1highThy-1high (B-type), and Sca-1lowThy-1low (C-type). We then performed microarray analysis to identify specific genes for each subpopulation.
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| Overall design |
To obtain fibroblast subpopulations, pulmonary single cells (Pu) were incubated with antibodies against lineage-specific cell surface markers (CD31, CD45, CD146, Lyve-1, TER119, and E-Cadherin), Pdgfra, Sca-1, and Thy-1. 50,000 cells of subpopulations (A-, B-, and C-type fibroblasts) in addition to Pu were isolated, and then RNA was extracted from each population and amplified for the gene expression comparison.
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| Contributor(s) |
Iwashita T, Matsushima S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 31, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Toshihide Iwashita |
| E-mail(s) |
toshiiwa@hama-med.ac.jp
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| Phone |
+81-53-435-2223
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| Organization name |
Hamamatsu University School of Medicine
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| Department |
Department of Regenerative and Infectious Pathology
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| Street address |
1-20-1 Handayama, Higashi-ku
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| City |
Hamamatsu city |
| State/province |
Shizuoka |
| ZIP/Postal code |
431-3192 |
| Country |
Japan |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA396506 |
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