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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 16, 2017 |
| Title |
The plasticizer Bisphenol A favors cancer progression in adult zebrafish by perturbing the epigenome: A systems level analysis of the miRNome (miRNA). |
| Organism |
Danio rerio |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Exposure to bisphenol A (BPA), an endocrine disruptor (ED), has raised concerns for both human and ecosystem health. Epigenetic factors, including microRNAs, are key regulators of gene expression during cancer. The effect of BPA exposure on the zebrafish epigenome remains poorly characterized. Zebrafish represents an excellent model to study cancer as the organism develops disease that resembles human cancer. Using zebrafish as systems toxicology model, we hypothesized that chronic BPA-exposure impacts the miRNome in adult zebrafish and establishes an epigenome more susceptible to cancer development. After a 3 week exposure to 100 nM BPA, RNA from the liver was extracted to perform high throughput mRNA and miRNA sequencing. Differential expression (DE) analyses comparing BPA-exposed to control specimens were performed using established bioinformatics pipelines. In the BPA-exposed liver, 6,188 mRNAs and 15 miRNAs were differently expressed (q ≤ 0.1). By analyzing human orthologs of the DE zebrafish genes, signatures associated with non-alcoholic fatty liver disease (NAFLD), oxidative phosphorylation, mitochondrial dysfunction and cell cycle were uncovered. Chronic exposure to BPA has a significant impact on the liver miRNome in adult zebrafish and has the potential to cause adverse outcomes including cancer.
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| Overall design |
Examination of miRNome changes in an in vivo model organism exposed to a common, environmental compound.
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| Contributor(s) |
Ludivine R, Hardiman G |
| Citation(s) |
29027980 |
| Submission date |
Jul 31, 2017 |
| Last update date |
Mar 26, 2021 |
| Contact name |
Gary Hardiman |
| E-mail(s) |
hardiman@musc.edu, g.hardiman@qub.ac.uk, glen@musc.edu
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| Organization name |
Medical University of South Carolina
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| Department |
Medicine
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| Street address |
135 Cannon Street
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| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
SC |
| Country |
USA |
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| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA396509 |
| SRA |
SRP114397 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE102059_Liver-BPA_vs_Cntrl-Liver_miRNA_DE.txt.gz |
10.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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