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Series GSE102128 Query DataSets for GSE102128
Status Public on Feb 27, 2018
Title RNA-seq of hiPSCs-derived NPCs from 3 pairs of dizygotic discordant twins for Congenital Zika syndrome
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Congenital Zika syndrome (CZS), caused by Zika virus (ZIKV) infection, has been associated to impairment of early brain development, particularly related to neural progenitor cells (NPCs) survival and growth. In this work we report in a high-throughput manner (RNA-Seq) the differences in the transcriptomes of hiPSCs(human induced pluripotent stem cells)-derived NPCs from 3 pairs of discordant twins for Congenital Zika syndrome (CZS). We found significant differences in the expression levels of genes relevant to the regulation of neural development between the NPCs from CZS-affected and non-affected twins, suggesting that epigenetic differences may contribute to the different susceptibilities of NPCs to ZIKV infection.
 
Overall design hiPSCs were generated from CD71+-cells, which were isolated from peripheral blood samples from three pairs of dizygotic/discordant twins. Then, NPCs were obtained after hiPSCs differentiation and cultivated for 4 days before the RNA was extracted and deep sequenced using Illumina HiSeq4000.
 
Contributor(s) Sena Amaral M, daSilva LF, Verjovski-Almeida S, Zatz M
Citation(s) 29396410
Submission date Aug 01, 2017
Last update date May 15, 2019
Contact name Murilo Sena Amaral
E-mail(s) sena@iq.usp.br
Organization name Universidade de São Paulo
Street address Avenida Prof Lineu Prestes
City São Paulo
State/province SP
ZIP/Postal code 05508000
Country Brazil
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (6)
GSM2724548 SVA-ZN1
GSM2724549 SVA-ZN3
GSM2724550 SVA-ZN5
Relations
BioProject PRJNA396752
SRA SRP114529

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE102128_count_table_zn.txt.gz 484.3 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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