 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 26, 2018 |
| Title |
Recapitulation of Human Neural Microenvironment Signatures in iPSC-Derived NPC 3D Differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Brain microenvironment plays an important role in neurodevelopment and function, where extracellular matrix (ECM) components and soluble factors modulate cellular features, as migration, proliferation survival and neuronal function. Disruption of microenvironment’s homeostasis is often related to pathological conditions. Here, we addressed the microenvironment remodeling occurring during in vitro differentiation of human neural stem cells (NSC) in a three-dimensional (3D) culture system. Proteome and transcriptome dynamics revealed significant changes namely at cell membrane and ECM composition during 3D differentiation, diverging significantly from the profile of monolayer cultures (2D). Structural proteoglycans typically found in brain ECM were enriched during 3D differentiation, while 2D cultures presented increased levels of basement membrane constituents (e.g., laminins, collagens and fibrillins). Moreover, higher expression levels of synaptic machinery and ion transport machinery constituents observed for 3D cultures, both at mRNA and protein levels, suggested a higher degree of neuronal maturation and organization relative to 2D differentiation. This work demonstrated that neural cellular and extracellular features can be recapitulated in the presented 3D neural cell model, highlighting its value to address molecular defects in cell-ECM interactions associated with neurological disorders.
|
| |
|
| Overall design |
mRNA profiles of day 0, 12 ad 30 of hiPSC-NSC 3D differentiation were generated by RNA sequencing for two hiPSC-NSC lines in duplicates, using Illumina HiSeq 4000.
|
| |
|
| Contributor(s) |
Simão D, Silva MM, Terrasso AP, Arez F, Sousa MF, Gomes-Alves P, Raimundo N, Alves PM, Brito C |
| Citation(s) |
30057262 |
| Submission date |
Aug 01, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Daniel Simão |
| E-mail(s) |
dsimao@itqb.unl.pt
|
| Organization name |
iBET, Instituto de Biologia Experimental e Biológica
|
| Street address |
Av. República, Qta. do Marquês, Estação Agronómica Nacional, Edificio IBET/ITQB
|
| City |
Oeiras |
| ZIP/Postal code |
2780-157 Oeiras |
| Country |
Portugal |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA396881 |
| SRA |
SRP114672 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE102139_hiPSC-NSC_3D_diff_processed.txt.gz |
2.1 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...