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| Status |
Public on Aug 01, 2019 |
| Title |
Transcriptional changes in mouse lungs induced by house dust mite |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We examined the transcriptional changes in mouse lungs induced by house dust mite (HDM), D.f. extract and the isolated RNA was utilized for RNA-seq analysis. Our results demonstrated that HDM strongly triggered an inflammatory response.
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| Overall design |
Total RNA was isolated from the lung of wild type or TLR3MAVS double knockout mice that were treated with PBS or HDM (D.f.) extract for 14-16 hours. Illumina TruSeq RNA Sample Preparation was performed following manufacturer's protocol. Samples were run on an Illumina HiSeq 3000 . Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm9 whole genome using TopHat v2.0.8b. Genes were annotated and quantified by HTSeq-DESeq pipeline.
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| |
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| Contributor(s) |
Zou Y, Chen Y, Li X |
| Citation(s) |
31562213 |
| Submission date |
Aug 03, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Yidong Chen |
| E-mail(s) |
cheny8@uthscsa.edu
|
| Phone |
2105629163
|
| Organization name |
UT Health Science Center at San Antonio
|
| Department |
Population Health Sciences
|
| Street address |
8403 Floyd Curl Drive, MSC 7784
|
| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE102212 |
Identification and Characterization of House Dust Mites-associated Immunogenic RNA |
|
| Relations |
| BioProject |
PRJNA397020 |
| SRA |
SRP114764 |
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