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| Status |
Public on Aug 08, 2017 |
| Title |
Biological and RNA Regulatory Function of MOV10 in Mammalian Germ Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Background: RNA regulation by RNA-binding proteins (RBPs) involve extremely complicated mechanisms. MOV10 and MOV10L1 are two homologous RNA helicases implicated in distinct intracellular pathways. MOV10L1 participates specifically in Piwi-interacting RNA (piRNA) biogenesis and protects mouse male fertility. In contrast, the functional complexity of MOV10 remains incompletely understood, and its role in the mammalian germline is unknown. Here we report a study of the biological and molecular functions of the RNA helicase MOV10 in mammalian male germ cells.
Results: MOV10 is a nucleocytoplasmic protein mainly expressed in spermatogonia. Knockdown and transplantation experiments show that MOV10 deficiency has a negative effect on spermatogonial progenitor cells (SPCs), limiting proliferation and in vivo repopulation capacity. This effect is concurrent with a global disturbance of RNA homeostasis and downregulation of factors critical for SPC proliferation and/or self-renewal. Unexpectedly, microRNA (miRNA) biogenesis is impaired due partially to decrease of miRNA primary transcript levels and/or retention of miRNA via splicing control. Genome-wide analysis of RNA targetome reveals that MOV10 binds preferentially to mRNAs with long 3'-UTR, and also interacts with various non-coding RNA species including those in the nucleus. Intriguingly, nuclear MOV10 associates with an array of splicing factors, particularly with SRSF1, and its intronic binding sites tend to reside in proximity to splice sites.
Conclusions: These data expand the landscape of MOV10 function and highlight a previously unidentified role initiated from the nucleus, suggesting that MOV10 is a versatile RBP involved in a broader RNA regulatory network.
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| Overall design |
Triplicate identification of transcripts associated with MOV10 in mice testes, triplicate mRNA profiles and miRNA profile of Mov10 knockdown and control in spermatogonial progenitor cells were generated by deep sequencing.
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| Contributor(s) |
Fu K, Tian S, Tan H, Wang C, Wang H |
| Citation(s) |
31088452 |
| Submission date |
Aug 07, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Suwen Tian |
| E-mail(s) |
tiansuwen116@163.com
|
| Phone |
86-15950528893
|
| Organization name |
Nanjing Medical University
|
| Street address |
101Longmian Avenue
|
| City |
Nanjing |
| ZIP/Postal code |
211100 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA397383 |
| SRA |
SRP114934 |
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