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Series GSE10239 Query DataSets for GSE10239
Status Public on Jan 23, 2008
Title Functional and Genomic Profiling of Effector CD8 T Cell Subsets
Organism Mus musculus
Experiment type Expression profiling by array
Summary Using killer cell lectin-like receptor G1 as a marker to distinguish terminal effector cells from memory precursors, we found that despite their diverse cell fates both subsets possessed remarkably similar gene expression profiles and functioned as equally potent killer cells. However, only the memory precursors were capable of making IL-2 thus defining a novel effector cell that was cytotoxic, expressed granzyme B, and produced inflammatory cytokines in addition to IL-2. This effector population then differentiated into long-lived protective memory T cells capable of self-renewal and rapid re-call responses. Mechanistic studies showed that cells that continued to receive antigenic stimulation during the later stages of infection were more likely to become terminal effectors. Importantly, curtailing antigenic stimulation towards the tail-end of the acute infection enhanced the generation of memory cells. These studies support the decreasing potential model of memory differentiation and show that the duration of antigenic stimulation is a critical regulator of memory formation
Keywords: Infection Type
 
Overall design An important question in memory development is understanding the differences between effector CD8 T cells that die versus effector cells that survive and give rise to memory cells. In this study we provide a comprehensive phenotypic, functional and genomic profiling of terminal effectors and memory precursors, towards better understanding the generation of these subsets. The two effector subsets were FACS purified during the early expansion phase (Days 4-5 post-infection) and extensively analyzed for their phenotypic (eg. CD127, CD62L, CD27, Bcl-2, Granzyme B, etc.) and functional properties (cytokine production, cytotoxicity, homeostatic proliferation, recall proliferation), and gene expression profiles (by genome-wide microarray analyses). Mechanistic studies involving the extent of proliferation and duration of antigen stimulation on memory differentiation potential of effectors were also performed using adoptive transfer techniques.
 
Citation(s) 18316415
Submission date Jan 22, 2008
Last update date Feb 11, 2019
Contact name Shruti Subramaniam
Organization name Emory University
Department Emory University School of Medicine
Street address G211 RRC, Emory University School of Medicine
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM257826 CD8 P14 Naive cells-1
GSM257827 CD8 P14 Naive cells-2
GSM257828 CD8 P14 Naive cells-3
Relations
BioProject PRJNA108417

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10239_RAW.tar 45.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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