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| Status |
Public on Feb 06, 2018 |
| Title |
Tacrolimus-induced nephrotoxicity in mice is associated with microRNA deregulation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
|
| Summary |
Although Tacrolimus is an immunosuppressive drug widely used in renal transplantation, its chronic use paradoxically induces nephrotoxic effects, in particular renal fibrosis, which is responsible for chronic allograft dysfunction and represents a major prognostic factor of allograft survival. As molecular pathways and mechanisms involved in Tacrolimus-induced fibrogenic response are poorly elucidated, we assessed whether miRNAs are involved in the nephrotoxic effects mediated by Tacrolimus. Treatment of CD-1 mice with Tacrolimus (1mg/kg/d for 28 days) resulted in kidney injury and was associated with alteration of a gene expression signature associated with cellular stress, fibrosis and inflammation. Tacrolimus also affected renal miRNA expression, including miRNAs previously involved in fibrotic and inflammatory processes including “fibromirs” such as miR-21-5p. Specifically, Renal Proximal Tubular Epithelial cells exposed to tacrolimus showed up-regulation of miR-21-5p and the concomitant induction of epithelial phenotypic changes, inflammation and oxidative stress. In conclusion, this study suggests for the first time that miRNAs, especially fibromiRs, mediate Tacrolimus-induced nephrotoxic effects.
This SuperSeries is composed of the SubSeries listed below.
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| Overall design |
Refer to individual Series
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| |
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| Contributor(s) |
Cauffiez C, Savary G, Nottet N, Pottier N, Mari B |
| Citation(s) |
29362864 |
| Submission date |
Aug 10, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
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| Platforms (2) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
| GPL23897 |
Agilent-031184 Unrestricted Mouse miRNA V21 Microarray (Feature Number version) |
|
| Samples (16)
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| This SuperSeries is composed of the following SubSeries: |
| GSE102513 |
Tacrolimus-induced nephrotoxicity in mice is associated with microRNA deregulation [transcriptome analysis] |
| GSE102514 |
Tacrolimus-induced nephrotoxicity in mice is associated with microRNA deregulation (miRNome analysis) |
|
| Relations |
| BioProject |
PRJNA397918 |
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