|
| Status |
Public on Jun 22, 2018 |
| Title |
Prep1 and Pbx2/4 dependent genes in zebraffish embryos |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Identification of Prep1 and Pbx2/4 regulated genes during early zebrafish embryogenesis (6 and 12hpf) by RNA-seq
|
| |
|
| Overall design |
zebrafish embryos were injected with morpholinos targeting Prep1, Pbx2/4 (TALE MO) mixed with water and red phenol dye or injected with water and red phenol dye alone (Control). Then total RNA was extracted from 6 or 12hpf injected embryos and polyA+RNA libraries constructed using the TruSeq stranded RNAseq LT kit from illumina
|
| |
|
| Contributor(s) |
Ladam FG, Stanney W, Donaldson IJ, Bobola N, Sagerstrom CG |
| Citation(s) |
29911973 |
| Submission date |
Aug 15, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Franck Ladam |
| E-mail(s) |
franck.ladam@umassmed.edu
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Biochemistry and Molecular Pharmacology
|
| Lab |
Charles Sagerstrom
|
| Street address |
364 plantation street , LRB 870D
|
| City |
Worcester |
| State/province |
Massachusetts |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
|
| Samples (12)
|
| GSM2742591 |
Control zebrafish 6hpf biological replicate1 |
| GSM2742592 |
Control zebrafish 6hpf biological replicate2 |
| GSM2742593 |
Control zebrafish 6hpf biological replicate3 |
| GSM2742594 |
TALE MO zebrafish 6hpf biological replicate1 |
| GSM2742595 |
TALE MO zebrafish 6hpf biological replicate2 |
| GSM2742596 |
TALE MO zebrafish 6hpf biological replicate3 |
| GSM2742597 |
Control zebrafish 12hpf biological replicate1 |
| GSM2742598 |
Control zebrafish 12hpf biological replicate2 |
| GSM2742599 |
Control zebrafish 12hpf biological replicate3 |
| GSM2742600 |
TALE MO zebrafish 12hpf biological replicate1 |
| GSM2742601 |
TALE MO zebrafish 12hpf biological replicate2 |
| GSM2742602 |
TALE MO zebrafish 12hpf biological replicate3 |
|
| Relations |
| BioProject |
PRJNA398335 |
| SRA |
SRP115456 |
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