|
| Status |
Public on Feb 12, 2018 |
| Title |
A requirement for dual-specificity phosphatase 6 in zebrafish gametogenesis |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Identification of genes that are differentially-expressed in dusp2um287/um287;dusp6um286/um286 mutant embryos compared to wildtype
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| |
|
| Overall design |
Total RNA was extracted from pools of dechrionated, deyolked wildtype and dusp2um287/um287;dusp6um286/um286 embryos at 18hpf using the RNeasy Mini Kit (Qiagen). Three libraries from wildtype embryos and three libraries from dusp2um287/um287;dusp6um286/um286 embryos were then generated from 3mg RNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina). All libraries were analyzed for quality on a bioanalyzer prior to sequencing (Agilent 2100 BioAnalyzer).
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| |
|
| Contributor(s) |
Maurer JM, Sagerstrom CG |
| Citation(s) |
29544468 |
| Submission date |
Aug 18, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jennifer Maurer |
| E-mail(s) |
jennifer.maurer@umassmed.edu
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Biochemistry and Molecular Pharmacology
|
| Lab |
Sagerstrom
|
| Street address |
364 Plantation Street, LRB 870D
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
|
| Samples (6)
|
| GSM2746500 |
dusp2um287/um287;dusp6um286/um286 mutant replicate 1 |
| GSM2746501 |
dusp2um287/um287;dusp6um286/um286 mutant replicate 2 |
| GSM2746502 |
dusp2um287/um287;dusp6um286/um286 mutant replicate 3 |
|
| Relations |
| BioProject |
PRJNA399093 |
| SRA |
SRP115796 |
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