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Series GSE10293 Query DataSets for GSE10293
Status Public on Jan 29, 2008
Title Rat expression data after acute FSH suppression
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary To unravel the genes that underpin the effects of FSH on spermatogenesis, testicular gene expression was compared in juvenile rats after FSH suppression to littermate controls. Based on existing data, it is apparent that the dominant function of FSH shifts between 9 and 18 day postpartum (dpp) during the first wave of spermatogenesis from driving Sertoli cell proliferation to support of germ cells. To enable comprehensive analysis of the impact of acute in vivo FSH suppression on Sertoli and germ cell development and to determine the genes that underpin these affects, FSH was selectively suppressed in Sprague Dawley rats by passive immunisation for 4 days prior to testis collection at 18 days postpartum. FSH suppression did not affect Sertoli cell proliferation at 18 dpp, but germ cell numbers were decreased at 18 dpp following FSH suppression, with a corresponding increase in germ cell apoptosis measured. Sixty transcripts were measured as changed at 18 dpp in response to 4 days of FSH suppression, as assessed using Affymetrixâ„¢ microarrays. Some of these are known as Sertoli cell-derived FSH-responsive genes (e.g. StAR, cathepsin L, insulin-like growth factor binding protein-3), while others encode proteins involved in cell cycle and survival regulation (e.g. cyclin D1, scavenger receptor class B 1). These data demonstrate that FSH affects germ cells in vivo, by supporting germ cell viability at day 18. This model enabled identification of candidate genes that contribute to the FSH-mediated pathway by which Sertoli cells support germ cells.
Keywords: time course
Overall design Sprague Dawley male rats at day 14 postpartum received either a rat FSH antibody raised in sheep or a control sheep immunoglobulin for 4 days. RNA was extracted from the testis and hybridisation on Affymetrix microarrays was conducted.
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Contributor(s) Meachem S, Ruwanpura S, Ziolkowski J, Ague J, Skinner M, Loveland K
Citation(s) 16135663
Submission date Jan 28, 2008
Last update date Feb 21, 2017
Contact name Michael K Skinner
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
Platforms (1)
GPL85 [RG_U34A] Affymetrix Rat Genome U34 Array
Samples (4)
GSM259915 Control IgG for 4 days replicate 1
GSM259916 Control IgG for 4 days replicate 2
GSM259917 FSH antibody for 4 days replicate 1
BioProject PRJNA108507

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Supplementary file Size Download File type/resource
GSE10293_RAW.tar 8.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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