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| Status |
Public on Jul 15, 2019 |
| Title |
Divergent lncRNA MYMLR regulates MYC by eliciting DNA looping and promoter-enhancer interaction |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Long non-coding RNAs (lncRNAs) function in a wide range of processes by diverse mechanisms, though their roles in regulation of oncogenes and/or tumor suppressors remain rather elusive. We performed a global search for lncRNAs affecting MYC activity using a systems biology-based approach with a K supercomputer and the GIMLET algorism based on local distance correlations. Consequently, MYMLR was identified and experimentally shown to maintain MYC transcriptional activity and cell cycle progression despite the low levels of expression. A proteomic search for MYMLR-binding proteins identified PCBP2, while it was also found that MYMLR places a 557-kb upstream enhancer region in the proximity of the MYC promoter in cooperation with PCBP2. These findings implicate a crucial role for MYMLR in regulation of the archetypical oncogene MYC and warrant future studies regarding the involvement of low copy number lncRNAs in regulation of other crucial oncogenes and tumor suppressor genes.
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| Overall design |
Microarray analysis using a SurePrint G3 Human GE 8 x 60K v2 Microarray G4851B (Agilent) was conducted
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| Contributor(s) |
Kajino T, Shimamura T, Gong S, Yanagisawa K, Ida L, Nakatochi M, Griesing S, Shimada Y, Kano K, Suzuki M, Miyano S, Takahashi T |
| Citation(s) |
31361039 |
| Submission date |
Aug 25, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Takashi Takahashi |
| Organization name |
Aichi Cancer Center
|
| Street address |
1-1 Kanokoden, Chikusa-ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
464-8681 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA400173 |
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