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| Status |
Public on Aug 26, 2017 |
| Title |
Vegetally localised Vrtn functions as a novel repressor to modulate bmp2b transcription during dorsoventral patterning in zebrafish |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The vegetal pole cytoplasm represents a crucial source of maternal dorsal determinants for patterning the dorsoventral axis of the early embryo. Removal of the vegetal yolk in the zebrafish fertilised egg before the completion of the first cleavage results in embryonic ventralisation, but removal of this part at the two-cell stage leads to embryonic dorsalisation. How this is achieved remains unknown. Here, we report a novel mode of maternal regulation of BMP signaling during dorsoventral patterning in zebrafish. We identify Vrtn as a novel vegetally localised maternal factor with dorsalising activity and rapid transport towards the animal pole region after fertilisation. Co-injection of vrtn mRNA with vegetal RNAs from different cleavage stages suggests the presence of putative vegetally localised Vrtn antagonists with slower animal pole transport. Thus, vegetal ablation at the two-cell stage could remove most of the Vrtn antagonists, and allows Vrtn to produce the dorsalising effect. Mechanistically, Vrtn binds a bmp2b regulatory sequence and acts as a repressor to inhibit its zygotic transcription. Analysis of maternal-zygotic vrtn mutants further shows that Vrtn is required to constrain excessive bmp2b expression in the margin. Our work unveils a novel maternal mechanism regulating zygotic BMP gradient in dorsoventral patterning.
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| Overall design |
RNA seq of animal pole and vegetal pole, respectively.
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| Contributor(s) |
Shao M, Shi D |
| Citation(s) |
28928283 |
| Submission date |
Aug 25, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Ming Shao |
| E-mail(s) |
shaoming@sdu.edu.cn
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| Phone |
86 13853136468
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| Organization name |
Shandong University
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| Department |
School of Lifesciences
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| Street address |
27# Shan Da Nan Lu Road
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| City |
Jinan |
| State/province |
Shandong |
| ZIP/Postal code |
250100 |
| Country |
China |
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| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA400171 |
| SRA |
SRP116182 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE103090_SMA3_vs_SMV3.edgeR.significant.diff.txt.gz |
53.0 Kb |
(ftp)(http) |
TXT |
| GSE103090_all.rpkm.txt.gz |
642.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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