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| Status |
Public on Nov 13, 2017 |
| Title |
Identification of quiescence and early activation histones modifications pattern in satellite cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report that the current stem cell isolation procedures induce important transcriptional and epigenetic modifications. Focusing on adult skeletal muscle stem cells (MuSCs), here we report the epigenome (Histone 3 modifications by ChIP-sequencing) of true quiescent MuSCs and compare it to early-activated MuSCs isolated by the conventional method.
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| Overall design |
Histone 3 modifications (H3K4me3, H3K27ac, H3K27me3) ChIP-seq analysis of muscle stem cells that were either fixed in situ (T0) or isolated with the traditional dissociation protocol (T3).
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| Contributor(s) |
Machado L, Esteves de Lima J, Fabre O, Proux C, Legendre R, Szegedi A, Varet H, Ingerslev L, Barrès R, Relaix F, Mourikis P |
| Citation(s) |
29141227 |
| Submission date |
Aug 28, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Rachel Legendre |
| E-mail(s) |
rachel.legendre@pasteur.fr
|
| Organization name |
Institut Pasteur
|
| Department |
Research and Resource Center for Scientific Informatics
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| Lab |
Hub of Bioinformatics and Biostatistics
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| Street address |
28, rue du docteur Roux
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| City |
Paris |
| ZIP/Postal code |
75724 |
| Country |
France |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA400385 |
| SRA |
SRP116305 |
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