|
| Status |
Public on Dec 12, 2017 |
| Title |
Analysis of gene expression in the rel- + relA+ and rel- + relAE335Q strains of Synechococcus elongatus PCC7942 with RNA sequencing during constant light conditions |
| Organism |
Synechococcus elongatus PCC 7942 = FACHB-805 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We investigated the role of the basal levels of ppGpp in Synechococcus elongatus PCC7942 in constant light. We performed RNA-seq experiments using the rel- + relA+ and rel- + relAE335Q strains and show that basal levels of ppGpp are required for regulation of global transcription in light.
|
| |
|
| Overall design |
Enriched mRNA was prepared from synchronized S. elongatus cells at indicated time points using the Illumina TruSeq Stranded mRNA Sample Prep Kit and Illumina HiSeq technology.
|
| |
|
| Contributor(s) |
Puszynska AM, O'Shea EK |
| Citation(s) |
29241543 |
| Submission date |
Sep 05, 2017 |
| Last update date |
Jul 20, 2022 |
| Contact name |
Anna M Puszynska |
| E-mail(s) |
apuszyns@fas.harvard.edu
|
| Organization name |
Harvard University
|
| Department |
Molecular and Cellular Biology
|
| Lab |
O'Shea
|
| Street address |
52 Oxford St, Room 445
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16957 |
Illumina HiSeq 2000 (Synechococcus elongatus PCC 7942) |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE103606 |
ppGpp controls global gene expression in light and in darkness in S. elongatus |
|
| Relations |
| BioProject |
PRJNA401777 |
| SRA |
SRP117071 |
Less...
More...