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| Status |
Public on Jul 12, 2018 |
| Title |
Whole genome bisulphite sequencing using the Illumina X system [Infinium MethylationEPIC] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
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| Summary |
While Illumina X technology has helped to reduce the cost of whole genome sequencing substantially, its application for bisulphite sequencing is not straightforward. We describe the optimization of a library preparation and sequencing approach that maximizes the yield and quality of sequencing, and how to eliminate a previously unrecognized artefact affecting several percent of bisulphite sequencing reads.
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| Overall design |
NA12878 and IMR90 cell ines assayed with Illumina Infinium MethylationEPIC BeadChip for comparison against whole genome bisulphite sequencing using BS-tagging protocol
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| Contributor(s) |
Suzuki M, Liao W, Wos F, Johnston AD, DeGrazia J, Ishii J, Bloom T, Zody MC, Germer S, Greally JM |
| Citation(s) |
30093547 |
| Submission date |
Sep 05, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Andrew Johnston |
| E-mail(s) |
Andrew.Johnston@med.einstein.yu.edu
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| Organization name |
Albert Einstein College of Medicine
|
| Department |
Genetics
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| Lab |
Price 314
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| Street address |
1301 Morris Park Avenue
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| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
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| Platforms (1) |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE103505 |
Whole genome bisulphite sequencing using the Illumina X system |
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| Relations |
| BioProject |
PRJNA401771 |
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