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Series GSE103503 Query DataSets for GSE103503
Status Public on Jul 12, 2018
Title Whole genome bisulphite sequencing using the Illumina X system [Bisulfite-Seq]
Organism Homo sapiens
Experiment type Methylation profiling by high throughput sequencing
Summary While Illumina X technology has helped to reduce the cost of whole genome sequencing substantially, its application for bisulphite sequencing is not straightforward. We describe the optimization of a library preparation and sequencing approach that maximizes the yield and quality of sequencing, and how to eliminate a previously unrecognized artefact affecting several percent of bisulphite sequencing reads.
 
Overall design Whole genome bisulphite sequencing of NA12878 and IMR90 cell lines using BS-tagging protocol
 
Contributor(s) Suzuki M, Liao W, Wos F, Johnston AD, DeGrazia J, Ishii J, Bloom T, Zody MC, Germer SM, Greally JM
Citation(s) 30093547
Submission date Sep 05, 2017
Last update date Jul 25, 2021
Contact name Andrew Johnston
E-mail(s) Andrew.Johnston@med.einstein.yu.edu
Organization name Albert Einstein College of Medicine
Department Genetics
Lab Price 314
Street address 1301 Morris Park Avenue
City Bronx
State/province NY
ZIP/Postal code 10461
Country USA
 
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (2)
GSM2772524 NA12878 WGBS
GSM2772525 IMR90 WGBS
This SubSeries is part of SuperSeries:
GSE103505 Whole genome bisulphite sequencing using the Illumina X system
Relations
BioProject PRJNA401763
SRA SRP116948

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE103503_RAW.tar 14.0 Gb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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