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Series GSE103671 Query DataSets for GSE103671
Status Public on Feb 15, 2019
Title Transcriptomic profiling of RV144 trial participants before and after vaccination with ALVAC-HIV and AIDSVAX B/E.[rv144pilot]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The RV144 clinical trial evaluated the efficacy of a vaccine regimen that included ALVAC-HIV prime and AIDSVAX boost in preventing HIV-1 acquisition. The vaccine reduced the risk of HIV-1 acquisition by 31.2%; however the mechanisms that led to the protection induced by this vaccine remain poorly understood. Our objectives were to identify transcriptional correlates and mechanisms that could explain the reduced acquisition conferred by the vaccine. We assessed the transcriptomic profile of HIV Env stimulated peripheral blood mononuclear cells collected from 223 participants two weeks after vaccination and from 40 placebo recipients.
 
Overall design Forty (40) participants of the RV144 clinical trial were primed twice with ALVAC-HIV (at week 0 and week 4) and boosted twice with AIDSVAX B/E in alum adjuvant (at week 12 and week 24). An additional ten (10) participants of the RV144 clinical trial received placebo at the same immunization timepoints (at week 0, week 4, week 12 and week 24). Blood samples were taken pre-vaccination and 2 weeks after the last immunization boost (week 26). All the participants remained HIV-1 negative at last follow-up (up to 3 years after vaccination). Peripheral blood mononuclear cells from those 50 participants was stimulated in vitro for 15 hours with Env peptides or with the vehicle (DMSO). RNA was then extracted and hybridized to Illumina beadchips.
 
Contributor(s) Fourati S, Sékaly R
Citation(s) 30787294
Submission date Sep 10, 2017
Last update date Jul 25, 2021
Contact name Slim Fourati
E-mail(s) slim.fourati@northwestern.edu
Organization name Northwestern University
Department Medicine
Lab Fourati
Street address 300 E Superior St
City Chicago
State/province Illinois
ZIP/Postal code 60611
Country USA
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (186)
GSM2778795 post-vaccination blood sample from donor 124184 stimulated 15 hours with 92TH023ENV
GSM2778796 pre-vaccination blood sample from donor 124184 stimulated 15 hours with 92TH023ENV
GSM2778797 pre-vaccination blood sample from donor 124184 stimulated 15 hours with DMSO
This SubSeries is part of SuperSeries:
GSE103740 Transcriptomic profiling of RV144 trial participants
Relations
BioProject PRJNA406919

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE103671_RAW.tar 415.6 Mb (http)(custom) TAR (of IDAT)
GSE103671_non-normalized.txt.gz 36.2 Mb (ftp)(http) TXT
Processed data included within Sample table
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