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| Status |
Public on Dec 12, 2017 |
| Title |
Analysis of gene expression in wild type and the rel- strain of Synechococcus elongatus PCC7942 - RNA sequencing during exposure to darkness (cells grown in light/dark conditions) |
| Organism |
Synechococcus elongatus PCC 7942 = FACHB-805 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We investigated the role of ppGpp in Synechococcus elongatus PCC7942 in light/dark conditions. We performed RNA-seq experiments using wild type and the rel- strain at dusk and during exposure to 12 h of darkness. We show that ppGpp is required for correct response to darkness.
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| Overall design |
Enriched mRNA was prepared from synchronized S. elongatus cells at indicated time points using the Illumina TruSeq Stranded mRNA Sample Prep Kit and Illumina HiSeq technology.
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| Contributor(s) |
Puszynska AM, O'Shea EK |
| Citation(s) |
29241543 |
| Submission date |
Sep 11, 2017 |
| Last update date |
Jul 20, 2022 |
| Contact name |
Anna M Puszynska |
| E-mail(s) |
apuszyns@fas.harvard.edu
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| Organization name |
Harvard University
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| Department |
Molecular and Cellular Biology
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| Lab |
O'Shea
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| Street address |
52 Oxford St, Room 445
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
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| Platforms (1) |
| GPL16957 |
Illumina HiSeq 2000 (Synechococcus elongatus PCC 7942) |
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| Samples (36)
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| GSM2779437 |
wild type replicate 1, dusk (light dark conditions) |
| GSM2779438 |
wild type replicate 1, dark, 5 min (light dark conditions) |
| GSM2779439 |
wild type replicate 1, dark, 15 min (light dark conditions) |
| GSM2779440 |
wild type replicate 1, dark, 30 min (light dark conditions) |
| GSM2779441 |
wild type replicate 1, dark, 1 h (light dark conditions) |
| GSM2779442 |
wild type replicate 1, dark, 2 h (light dark conditions) |
| GSM2779443 |
wild type replicate 1, dark, 4 h (light dark conditions) |
| GSM2779444 |
wild type replicate 1, dark, 8 h (light dark conditions) |
| GSM2779445 |
wild type replicate 1, dark, 12 h (light dark conditions) |
| GSM2779446 |
rel- replicate 1, dusk (light dark conditions) |
| GSM2779447 |
rel- replicate 1, dark, 5 min (light dark conditions) |
| GSM2779448 |
rel- replicate 1, dark, 15 min (light dark conditions) |
| GSM2779449 |
rel- replicate 1, dark, 30 min (light dark conditions) |
| GSM2779450 |
rel- replicate 1, dark, 1 h (light dark conditions) |
| GSM2779451 |
rel- replicate 1, dark, 2 h (light dark conditions) |
| GSM2779452 |
rel- replicate 1, dark, 4 h (light dark conditions) |
| GSM2779453 |
rel- replicate 1, dark, 8 h (light dark conditions) |
| GSM2779454 |
rel- replicate 1, dark, 12 h (light dark conditions) |
| GSM2779455 |
wild type replicate 2, dusk (light dark conditions) |
| GSM2779456 |
wild type replicate 2, dark, 5 min (light dark conditions) |
| GSM2779457 |
wild type replicate 2, dark, 15 min (light dark conditions) |
| GSM2779458 |
wild type replicate 2, dark, 30 min (light dark conditions) |
| GSM2779459 |
wild type replicate 2, dark, 1 h (light dark conditions) |
| GSM2779460 |
wild type replicate 2, dark, 2 h (light dark conditions) |
| GSM2779461 |
wild type replicate 2, dark, 4 h (light dark conditions) |
| GSM2779462 |
wild type replicate 2, dark, 8 h (light dark conditions) |
| GSM2779463 |
wild type replicate 2, dark, 12 h (light dark conditions) |
| GSM2779464 |
rel- replicate 2, dusk (light dark conditions) |
| GSM2779465 |
rel- replicate 2, dark, 5 min (light dark conditions) |
| GSM2779466 |
rel- replicate 2, dark, 15 min (light dark conditions) |
| GSM2779467 |
rel- replicate 2, dark, 30 min (light dark conditions) |
| GSM2779468 |
rel- replicate 2, dark, 1 h (light dark conditions) |
| GSM2779469 |
rel- replicate 2, dark, 2 h (light dark conditions) |
| GSM2779470 |
rel- replicate 2, dark, 4 h (light dark conditions) |
| GSM2779471 |
rel- replicate 2, dark, 8 h (light dark conditions) |
| GSM2779472 |
rel- replicate 2, dark, 12 h (light dark conditions) |
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| This SubSeries is part of SuperSeries: |
| GSE103606 |
ppGpp controls global gene expression in light and in darkness in S. elongatus |
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| Relations |
| BioProject |
PRJNA404081 |
| SRA |
SRP117265 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE103704_Expression.xls.gz |
946.9 Kb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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