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| Status |
Public on Sep 11, 2019 |
| Title |
Active fluctuations of the nuclear envelope shape the transcriptional dynamics in oocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Nucleus position in cells can act as a developmental cue. Mammalian oocytes position their nucleus centrally using an F-actin mediated pressure gradient. The biological significance of nucleus centring in mammalian oocytes being unknown, we sought to assess the F-actin pressure gradient effect on the nucleus. We addressed this using a dedicated computational 3D imaging approach, biophysical analyses, and a nucleus repositioning assay in mouse oocytes mutant for cytoplasmic F-actin. We found that the cytoplasmic activity, in charge of nucleus-centring, shaped the nucleus while promoting nuclear envelope fluctuations and chromatin motion. Off-centred nuclei in F-actin mutant oocytes were misshaped with immobile chromatin and modulated gene expression. Restoration of F-actin in mutant oocytes rescued nucleus architecture fully and gene expression partially. Thus, the F-actin mediated pressure gradient also modulates nucleus dynamics in oocytes. Moreover, this study supports a mechano-transduction model whereby cytoplasmic microfilaments could modulate oocyte transcriptome, essential for subsequent embryo development.
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| Overall design |
2 samples of Fmn2 +/- oocytes, 50 oocytes each and 2 samples of Fmn 2-/- oocytes, 50 oocytes each. For each biological sample, 3 technical replicates were done.
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| Contributor(s) |
Almonacid M, Al Jord A, El-Hayek S, Othmani A, Coulpier F, Lemoine S, Miyamoto K, Grosse R, Klein C, Piolot T, Mailly P, Voituriez R, Genovesio A, Verlhac M |
| Citation(s) |
31607652, 36038550 |
| Submission date |
Sep 11, 2017 |
| Last update date |
Sep 15, 2022 |
| Contact name |
Stephane Le Crom |
| Organization name |
IBENS
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| Department |
Genomic Core Facility
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| Street address |
46 rue Ulm
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| City |
PARIS |
| ZIP/Postal code |
75230 |
| Country |
France |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA406820 |
| SRA |
SRP117280 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE103718_RAW.tar |
2.2 Mb |
(http)(custom) |
TAR (of TAB) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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