|
| Status |
Public on Jul 11, 2018 |
| Title |
Characterization of imprinted methylation using the Illumina Infinium MethylationEPIC array platform |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
|
| Summary |
Bisulphite (BS) converted DNA from 2 paternal uniparental diploidies (pUPDs), one maternal (mUPD) and 5 control leukocytes samples were hybridized to the Infinium HumanMethylationEPIC BeadChip (Illumina), obtaining the BS DNA methylation profiles across approximately 850,000 CpGs. In addition, the 5 control leukocyte samples were also coverted using oxidative bisulphite (oxBS) treatment. The selective chemical oxidation of 5-hydroxymethylcytosine (5hmC) to 5-formylcytosine (5fC) and the deamination of the latter to uracil during the BS conversion allowed the quantification of independent 5-methylcytosine (5mC) and 5hmC methylation levels at every single CpG.
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| |
|
| Overall design |
Bisulphite converted DNA from 3 reciprocal uniparental diplodies were hybridised to the Infinium HumanMethylationEPIC BeadChip (Illumina) together with bisulphite and oxidative bisulphite converted DNA from 5 control leukocytes.
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| |
|
| Contributor(s) |
Hernandez JR, Monk D |
| Citation(s) |
29962238, 33053156 |
| Submission date |
Sep 12, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Jose Ramon Hernandez |
| Organization name |
Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)
|
| Department |
Cancer Epigenetics and Biology Program (PEBC)
|
| Lab |
Imprinting and Cancer group
|
| Street address |
Av. Gran Via de l'Hospitalet 199-203
|
| City |
L'Hospitalet de Llobregat, Barcelona |
| ZIP/Postal code |
08907 |
| Country |
Spain |
| |
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| Platforms (1) |
|
| Samples (13)
|
| GSM2780472 |
bisulphite converted DNA from control leukocytes 2 |
| GSM2780473 |
oxidative bisulphite converted DNA from control leukocytes 2 |
| GSM2780474 |
bisulphite converted DNA from control leukocytes 13 |
| GSM2780475 |
oxidative bisulphite converted DNA from control leukocytes 13 |
| GSM2780476 |
bisulphite converted DNA from control leukocytes 14 |
| GSM2780477 |
oxidative bisulphite converted DNA from control leukocytes 14 |
| GSM2780478 |
bisulphite converted DNA from control leukocytes 15 |
| GSM2780479 |
oxidative bisulphite converted DNA from control leukocytes 15 |
| GSM2780480 |
bisulphite converted DNA from control leukocytes 16 |
| GSM2780481 |
oxidative bisulphite converted DNA from control leukocytes 16 |
| GSM2780482 |
bisulphite converted DNA from paternal uniparental diplody 889 |
| GSM2780483 |
bisulphite converted DNA from paternal uniparental diplody 2705 |
| GSM2780484 |
bisulphite converted DNA from maternal uniparental diplody 1407 |
|
| Relations |
| BioProject |
PRJNA406898 |
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