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Series GSE10386 Query DataSets for GSE10386
Status Public on Mar 31, 2008
Title Co-activator function of RIP140 for NFκB/p65-dependent cytokine gene
Organism Mus musculus
Experiment type Expression profiling by array
Summary High- and low-grade inflammatory responses represent a hallmark of numerous pathologies such as sepsis and bacterial infection or insulin resistance and obesity, respectively. Here we describe an unexpected co-activator function of receptor interacting protein (RIP) 140 for
nuclear factor (NF) kB, a master transcriptional regulator of inflammation in multiple tissues. Genetic as well as acute deficiency of RIP140, which has been characterized as a co-repressor of various nuclear receptors on metabolic target genes, led to the inhibition of the proinflammatory
program in macrophages, mediated by RIP140’s direct impact on cytokine gene promoter activity. Intriguingly, RIP140’s co-activator function in this setting was found to rely on direct protein-protein interactions with the NFκB subunit p65 and histone acetylase CREB-binding protein (CBP). RIP140-dependent control of proinflammatory gene expression via p65/CBP may, therefore, represent a molecular rational for the cellular integration of metabolic and inflammatory pathways.
Keywords: expression profiling
 
Overall design Gene expression profiling was done
for macrophages from RIP140 knockout or wild-type mice.
RNA isolation, cDNA and cRNA synthesis, and hybridization to
arrays of type Mouse Genome 430 2.0 from Affymetrix
(Santa Clara, CA, USA) were performed according to manufacturer’s
recommendations. 3 arrays per genotype were hybridized.
Microarray data were analysed based on ANOVA using a commercial
software package (Micro Array Solution, version 1.0, SAS Institute,
Cary, NC, USA). Standard settings were used, except the following
specifications: log-linear mixed models were fitted for values of
perfect-matches, with genotype considered to be constant and the
array-id random. Custom CDF with Unigene based gene/transcript
definitions was used to annotate the arrays.
 
Contributor(s) Zschiedrich I, Hardeland U, Krones-Herzig A, Diaz M, Vegiopoulus A, Müggenburg J, Sombroek D, Hofmann T, Zawatzky R, Yu X, Gretz N, Christian M, White R, Parker M, Herzig S
Citation(s) 18469200
Submission date Feb 05, 2008
Last update date Mar 19, 2012
Contact name Inka Zschiedrich
Organization name German Cancer Research Center Heidelberg
Department Molecular Metabolic Control
Lab Molecular Metabolic Control
Street address Im NeuenheimerFeld 280
City Heidelberg
ZIP/Postal code 69120
Country Germany
 
Platforms (1)
GPL6456 Affymetrix GeneChip Mouse Genome 430 2.0 Array [CDF: Mm_UNIGENEG_8]
Samples (6)
GSM262529 RIP140 knockout 1
GSM262530 RIP140 knockout 2
GSM262531 RIP140 knockout 3
Relations
BioProject PRJNA108129

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10386_RAW.tar 36.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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