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Series GSE104033 Query DataSets for GSE104033
Status Public on Jul 04, 2018
Title The dual methyltransferase METTL13 targets N-terminus and Lys55 of eEF1A and regulates codon-specific translation rates
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Other
Summary Eukaryotic elongation factor 1 alpha (eEF1A) delivers aminoacyl-tRNA to the ribosome and thereby plays a key role in protein synthesis. Human eEF1A is post-translationally methylated on several lysine residues as well as on the N-terminus and until recently the responsible methyltransferases were largely elusive. Using mass spectrometry based screens, gene targeted cells and in vitro enzymology we identify the human methyltransferase like proteins 13 (METTL13), which contains two distinct methyltransferase domains, as an enzyme catalyzing both dimethylation of Lys55 and trimethylation of the N-terminus of eEF1A. Moreover, through ribosome profiling we demonstrate that loss of METTL13 function alters translation dynamics and results in changed translation rate of specific codons. In line with the established descriptive nomenclature for this type of enzymes we suggest that METTL13 is redubbed eEF1A dual methyltransferase (eEF1A-DMT / EEF1ADMT).
 
Overall design ribosome profiling and poly(A) RNA sequencing of wildtype and MELL13-KO cells. Libraries were prepared as triplicates, except for wt ribosome profiling libraries that are duplicates only.
 
Contributor(s) Jakobsson ME, Małecki JM, Halabelian L, Nilges BS, Pinto R, Kudithipudi S, Munk S, Davydova E, Zuhairi FR, Arrowsmith CH, Jeltsch A, Leidel SA, Olsen JV, Falnes PØ
Citation(s) 30143613
Submission date Sep 20, 2017
Last update date May 15, 2019
Contact name Benedikt Sebastian Nilges
E-mail(s) benedikt.nilges@mpi-muenster.mpg.de
Organization name Max Planck Institute for Molecular Biomedicine
Department RNA Biology Group
Street address Von-Esmarch-Straße 54
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platforms (1)
GPL15456 Illumina HiScanSQ (Homo sapiens)
Samples (11)
GSM2788001 METTL13_KO_RPF_replicate1
GSM2788002 METTL13_KO_RPF_replicate2
GSM2788003 METTL13_KO_RPF_replicate3
Relations
BioProject PRJNA408071
SRA SRP118278

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Supplementary file Size Download File type/resource
GSE104033_CountTable.csv.gz 347.1 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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