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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 07, 2017 |
| Title |
Genome-wide RNA-seq transcriptional analysis of splenocyte responses to Dengue virus infection in WT, Irf3(-/-)×Irf7(-/-) (DKO), Irf3-/-xIrf5-/-xIrf7-/- (TKO), and Ifnar1-/- (AB6) mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Interferon-regulatory factors (IRFs) are a family of transcription factors (TFs) that play critical roles in translating viral recognition into antiviral responses, including type I IFN production. Dengue virus (DENV) and other clinically important flaviviruses are controlled by functional type I interferon (IFN) responses. Using an experimental model of DENV infection that recapitulates key aspects of the human disease in mice, we demonstrate that while mice lacking the type I IFN receptor (Ifnar1-/-) succumb to DENV infection, mice that are deficient in IRF-3, IRF-5, and IRF-7 – the three transcription factors thought to regulate type I IFN production – survive DENV challenge. Genome-wide RNA-seq analysis of WT, Irf3(-/-)×Irf7(-/-) (DKO), Irf3-/-xIrf5-/-xIrf7-/- (TKO), and Ifnar1-/- (AB6) splenocytes identified minimal type I IFN production but a robust type II IFN (IFN-γ) response in DKO and TKO mice later shown to be dependent on IRF-1. These results reveal a key role for IRF-1 in antiviral defense by activating both type I and II IFN responses during DENV infection.
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| Overall design |
RNA-seq of total splenocytes from WT, Irf3(-/-)×Irf7(-/-) (DKO), Irf3-/-xIrf5-/-xIrf7-/- (TKO), and Ifnar1-/- (AB6) mice either mock infected or infected with Dengue virus for 6 or 24 hours.
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| Contributor(s) |
Carlin AF, Shresta S |
| Citation(s) |
29117564 |
| Submission date |
Sep 25, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Aaron F Carlin |
| E-mail(s) |
acarlin@health.ucsd.edu
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| Organization name |
University of California, San Diego
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| Department |
Pathology and Medicine
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| Lab |
Carlin
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| Street address |
9500 Gilman Drive GPL Rm 118
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| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92093-0640 |
| Country |
USA |
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| Platforms (1) |
| GPL18061 |
AB 5500xl-W Genetic Analysis System (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA411999 |
| SRA |
SRP118787 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE104189_DENV_IRFs_RPKM.txt.gz |
2.4 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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