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Series GSE104452 Query DataSets for GSE104452
Status Public on Dec 14, 2017
Title Gcn5 regulates FGF signaling outputs through c-Myc during early differentiation of embryoid bodies [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Precise control of gene expression during development is orchestrated by transcription factors and co-regulators including chromatin modifiers. How particular chromatin modifying enzymes affect specific developmental processes is not well defined. Here we report that Gcn5, a HAT essential for embryonic development, is largely required for c-MYC target gene transcription downstream of FGF signaling in early embryoid bodies (EBs). Gcn5 null EBs display deficient activation of ERK and p38, disorganized cytoskeletal networks, and compromised capacity to differentiate toward mesodermal and endodermal lineages. These findings establish a regulatory role for Gcn5 in execution of a critical signaling pathway during early development.
 
Overall design Day 5 embryoibodies (EBs) derived from Gcn5fx/fx (4_1, 6_1) and Gcn5-/- (4_C2, 6_C4) ES cells were subject to H3K9ac ChIP-seq experiments, in order to examine the changes in genomewide distribution of H3K9ac upon Gcn5 loss at early differentiation stage. ChIP DNA from 4 independent replicative experiments, including 2 replicates for each isolate pair (Gcn5fx/fx and Gcn5-/-), were used for deep sequencing.
 
Contributor(s) Wang L, Dent SY, Lu Y
Citation(s) 29249668
Submission date Sep 29, 2017
Last update date Jul 25, 2021
Contact name Li Wang
E-mail(s) wli2@mdanderson.org
Organization name UT-MD Anderson Cancer Center
Department Epigenetics and molecular carcinogenesis
Street address 1808 Park Rd 1C
City Smithville
State/province TX
ZIP/Postal code 78957
Country USA
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (24)
GSM2800724 4_1_CHIP_H3_1
GSM2800725 4_1_CHIP_H3_2
GSM2800726 4_1_CHIP_K9ac_1
This SubSeries is part of SuperSeries:
GSE104454 Gcn5 regulates FGF signaling outputs through c-Myc during early differentiation of embryoid bodies
Relations
BioProject PRJNA412661
SRA SRP119159

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104452_RAW.tar 1.1 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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