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| Status |
Public on Feb 16, 2018 |
| Title |
Gene expression data of gdT-derived iPSCs and validated iPS clone for pluritest |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
γδT cells constitute a small proportion of lymphocytes in peripheral blood. Unlike αβT cells, the anti-tumor activities are exerted through several different pathways in a MHC-unrestricted manner. Thus, immunotherapy using γδT cells is considered to be effective for various types of cancer. Occasionally, however, ex vivo expanded cells are not as effective as expected due to cell exhaustion. To overcome the issue of T-cell exhaustion, researchers have generated induced pluripotent stem cells (iPSCs) that harbor the same T-cell receptor (TCR) genes as their original T-cells, which provide nearly limitless sources for antigen-specific cytotoxic T lymphocytes (CTLs). However, these technologies have focused on αβT cells and require a population of antigen-specific CTLs, which are purified by cell sorting with HLA-peptide multimer, as the origin of iPS cells. In the present study, we aimed to develop an efficient and convenient system for generating iPSCs that harbor rearrangements of the TCRG and TCRD gene regions (γδT-iPSCs) without cell-sorting. We stimulated human whole peripheral blood mononuclear cell (PBMC) culture using Interleukin-2 and Zoledronate to activate γδT cells. Gene transfer into those cells with the Sendai virus vector resulted in γδT cell-dominant expression of exogenous genes. The introduction of reprogramming factors into the stimulated PBMC culture allowed us to establish iPSC lines. Around 70% of the established lines carried rearrangements at the TCRG and TCRD gene locus. The γδT-iPSCs could differentiate into hematopoietic progenitors. Our technology will pave the way for new avenues toward novel immunotherapy that can be applied for various types of cancer.
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| Overall design |
We extracted total RNAs from two gdT-derived iPSCs (46A1-s3, 46C2-s4) and validated iPS clone (409B2) and performed microarray for pluritest.
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| Contributor(s) |
Koyanagi-Aoi M, Taniguchi-Ikeda M, Aoi T |
| Citation(s) |
29164800 |
| Submission date |
Oct 05, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Michiyo Koyanagi-Aoi |
| E-mail(s) |
mkoyaoi@med.kobe-u.ac.jp
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| Organization name |
Kobe University
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| Street address |
Chuo-ku, Kusunoki-cho 7-5-1
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| City |
Kobe City |
| ZIP/Postal code |
650-0017 |
| Country |
Japan |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA413287 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE104605_RAW.tar |
6.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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