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| Status |
Public on Jan 01, 2018 |
| Title |
Ythdf2-mediated m6A mRNA clearance modulates neural development in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
We found that the proliferation and differentiation capabilities of NSPCs decrease significantly in Ythdf2 null mutants.To explore the underlying molecular mechanism, we performed transcriptomics and well-established m6A-methylome analyses of NSPCs dervied from wild type and Ythdf2-/- embryo brains. RNA-seq data revealed that expressions of genes enriched in neural development pathways were significantly disturbed. The inhibitory genes, like Flrt2, Ptprd, et al. in regulation of JAK-STAT cascade, which contributes to the neuroprotection and neurite outgrowth, showed increased gene expressions and m6A enrichment by m6A-seq. We identified that without the recognizing and binding of Ythdf2, the degradation of neuron differentiation related m6A-modified mRNAs were delayed in Ythdf2-/-, thereby disturbing the proliferation and differentiation of NSPCs. In summary, our findings uncovered that Ythdf2 modulates neural developmental via regulating the clearance of mRNA targets.
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| |
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| Overall design |
Examination of gene expression levels and m6A levels in mRNAs in wild type and Ythdf2 deficient mouse E14.5 derived NSPCs
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| |
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| Contributor(s) |
Zhao X, Pan Q, Shi H, Lu Z |
| Citation(s) |
29855337 |
| Submission date |
Oct 12, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Qingfei Pan |
| E-mail(s) |
Qingfei.Pan@stjude.org
|
| Phone |
1-901-356-6214
|
| Organization name |
St. Jude Children's Research Hospital
|
| Department |
Department of Computational Biology
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| Lab |
Yu Lab
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA414058 |
| SRA |
SRP119834 |
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