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| Status |
Public on Oct 14, 2017 |
| Title |
Sequential gene regulatory events leading to glucocorticoid-evoked apoptosis of CEM human leukemic cells [HG_U95Av2] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Glucocorticoid (GC)- sensitive cells are killed by receptor (GR)-filling concentrations of GC for a sufficient time.
We used 3 closely related clones (2 sensitive, 1 resistant) from the CEM line of ALL cells to test the hypothesis that over time, a distinct set of regulated genes would be found in sensitive clones, with cross-talk to other significant pathways
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| Overall design |
CEM C1-15(resistant), CEM C1-6 (spontaneous revertant to sensitive) are subclones of resistant clone CEM C1. CEM C7-14 is a subclone of sensitive clone CEM C7. C1 and C7 were cloned from the original CEM ALL line. Samples were obtained 2, 8, 12 an 20 hr after addition of 10E-6 M dexamethasone (Dex) to triplicate cultures, RNA extracted and analyzed for transcript expression levels
**Please note that the submitter was unable to locate the raw data CEL files. Thus raw data is not included in the current records.**
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| Contributor(s) |
Webb MS, Miller AL, Howard TL, Johnson BH, Chumakov S, Fofanov Y, Nguyen-Vu T, Lin CY, Thompson EB |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 13, 2017 |
| Last update date |
Dec 13, 2018 |
| Contact name |
Scott Andrew Ochsner |
| E-mail(s) |
sochsner@bcm.edu
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| Phone |
713-798-6227
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| Organization name |
Baylor College of Medicine
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| Department |
Molecular and Cellular Biology
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| Lab |
SPP: Signaling Pathways Project
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| Street address |
One Baylor Plaza
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL8300 |
[HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array |
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| Samples (72)
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| This SubSeries is part of SuperSeries: |
| GSE104932 |
Sequential gene regulatory events leading to glucocorticoid-evoked apoptosis of CEM human leukemic cells |
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| Relations |
| BioProject |
PRJNA414184 |
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