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| Status |
Public on Jul 27, 2018 |
| Title |
RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
TRIM25 E3 ubiquitin ligase is a new RNA-binding protein. We present the first high-throughput analysis of the molecular interactomes of TRIM25. We show that TRIM25 is a bona fide RNA-binding protein that interacts with numerous coding and non-coding transcripts. This suggests that TRIM25 could play a role in the regulation of RNA metabolism.
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| Overall design |
We performed UV crosslinking and immunoprecipitations in HeLa cells transiently expressing T7-tagged TRIM25, followed by high-throughput sequencing of TRIM25-associated RNAs (CLIP-seq). This was done in three biological replicates.
Please note that each processed data is genrated from Trim25 and Mock dataset pairs (e.g. e.g.Trim25_exp1_significant_clusters.gtf.gz generated from both Trim25 CLIP-seq exp1 and Mock CLIP-seq exp1 ) and linked to the corresponding Trim25 CLIP-seq sample records.
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| Contributor(s) |
Choudhury NR, Heikel G, Trubitsyna M, Kubik P, Nowak JS, Webb S, Granneman S, Spanos C, Rappsilber J, Castello A, Michlewski G |
| Citation(s) |
29117863 |
| Submission date |
Oct 13, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Shaun Michael Webb |
| E-mail(s) |
shaun.webb@ed.ac.uk
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| Organization name |
University of Edinburgh
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| Department |
Wellcome Trust Centre for Cell Biology
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| Lab |
Bioinformatics Core Facility
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| Street address |
2.21 Swann Building, Kings Buildings
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| City |
Edinburgh |
| ZIP/Postal code |
EH9 3JR |
| Country |
United Kingdom |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA414217 |
| SRA |
SRP119918 |
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