|
| Status |
Public on Feb 14, 2008 |
| Title |
Gene expression in rheumatoid arthritis synovial macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Macrophages from RA synovial fluids were compared to primary human blood-derived macrophages.
Keywords: disease state analysis
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| |
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| Overall design |
Macrophages from synovial fluids of five RA patients were isolated by ficoll density gradient centrifugation, followed by positive selection of CD14+ cells using magnetic beads. The patients’ diagnoses were determined by their physicians, in each case a Board-certified rheumatologist at the Hospital for Special Surgery, and were definite RA according to American College of Rheumatology criteria. De-identified samples were processed in an anonymous manner using a protocol approved by the Institutional Review Board of the Hospital for Special Surgery.
Human peripheral blood mononuclear cells were isolated from venous blood of independent healthy donors by centrifugation on a Ficoll density gradient. Monocytes were obtained from PBMCs using anti-CD14 magnetic beads, and were >96% pure as verified by flow cytometry. Monocytes were differentiated into macrophages by culturing in RPMI 1640 medium supplemented with 10% fetal bovine serum and M-CSF (20 ng/ml). The viability and purity of macrophages was comparable in all conditions
Gene expression was analyzed using Affymetrix microarrays and protocols.
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| |
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| Contributor(s) |
Ivashkiv LB, Antoniv TT, Hu X, Anna Y, Park-Min K |
| Citation(s) |
18345002 |
| Submission date |
Feb 12, 2008 |
| Last update date |
Dec 13, 2018 |
| Contact name |
Taras Antoniv |
| Organization name |
Hospital for Special Surgery
|
| Department |
Research
|
| Lab |
Lionel B. Ivashkiv, MD
|
| Street address |
535 East 70th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL8300 |
[HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA107955 |
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