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Series GSE10500 Query DataSets for GSE10500
Status Public on Feb 14, 2008
Title Gene expression in rheumatoid arthritis synovial macrophages
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Macrophages from RA synovial fluids were compared to primary human blood-derived macrophages.
Keywords: disease state analysis
Overall design Macrophages from synovial fluids of five RA patients were isolated by ficoll density gradient centrifugation, followed by positive selection of CD14+ cells using magnetic beads. The patients’ diagnoses were determined by their physicians, in each case a Board-certified rheumatologist at the Hospital for Special Surgery, and were definite RA according to American College of Rheumatology criteria. De-identified samples were processed in an anonymous manner using a protocol approved by the Institutional Review Board of the Hospital for Special Surgery.
Human peripheral blood mononuclear cells were isolated from venous blood of independent healthy donors by centrifugation on a Ficoll density gradient. Monocytes were obtained from PBMCs using anti-CD14 magnetic beads, and were >96% pure as verified by flow cytometry. Monocytes were differentiated into macrophages by culturing in RPMI 1640 medium supplemented with 10% fetal bovine serum and M-CSF (20 ng/ml). The viability and purity of macrophages was comparable in all conditions
Gene expression was analyzed using Affymetrix microarrays and protocols.
Contributor(s) Ivashkiv LB, Antoniv TT, Hu X, Anna Y, Park-Min K
Citation(s) 18345002
Submission date Feb 12, 2008
Last update date Dec 13, 2018
Contact name Taras Antoniv
Organization name Hospital for Special Surgery
Department Research
Lab Lionel B. Ivashkiv, MD
Street address 535 East 70th Street
City New York
State/province NY
ZIP/Postal code 10021
Country USA
Platforms (1)
GPL8300 [HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array
Samples (8)
GSM265020 rheumatoid arthritis 9252
GSM265036 rheumatoid arthritis 121102
GSM265361 rheumatoid arthritis 2-21
BioProject PRJNA107955

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Supplementary file Size Download File type/resource
GSE10500_RAW.tar 58.1 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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