|
| Status |
Public on Oct 16, 2018 |
| Title |
High-fidelity base editing mediated by Cpf1-cytidine deaminase fusion |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
C-to-T base editing mediated by CRISPR/Cas9 base editors (BEs) needs a G/C-rich PAM and the editing fidelity is compromised by unwanted indels and non-C-to-T substitutions. We developed CRISPR/Cpf1-based BEs to recognize a T-rich PAM and induce efficient C-to-T editing with few indels and/or non-C-to-T substitutions. The requirement of editing fidelity in therapeutic-related trials necessitates the development of CRISPR/Cpf1-based BEs, which also facilitates base editing in A/T-rich regions.
|
| |
|
| Overall design |
Examination of indels and base substitutions induced by the CRISPR/Cpf1 base editors
|
| |
|
| Contributor(s) |
Yang L, Chen J, Huang X, Yang B, Wang Y, Li X |
| Citation(s) |
29553573 |
| Submission date |
Oct 16, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Li Yang |
| E-mail(s) |
liyang_fudan@fudan.edu.cn
|
| Organization name |
Fudan University
|
| Department |
Institutes of Biological Sciences
|
| Street address |
131 Dong-An Road
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (40)
|
|
| Relations |
| BioProject |
PRJNA414402 |
| SRA |
SRP119999 |
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