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| Status |
Public on Jul 01, 2019 |
| Title |
DHX36 resolves G-rich structures in mRNA untranslated region to allow their translation [cPDS-RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
DHX36 is a ATP-dependent, 3´-5´ RNA helicase of the DEAH family. Previous publications reported this helicase to associate with AU-rich elements and to specifically unwind G-quadruplex structures. Here, we performed RNA-seq in triplicats of HEK293 wildtype cells and DHX36-KO-HEK293 cells to identify changes in RNA abundance upon presence or absence of this helicase. After sequencing and mapping to the human genome at nucleotide-resolution level we analysed the mRNA abundance of target mRNAs, identified in this study (see PAR-CLIP). Our analyses show that binding of DHX36 to 3´UTRS of target mRNAs significantly reduce their abundance.
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| Overall design |
Examination of RNA abundance in HEK293 wildtype cells and DHX36-KO-HEK293 cells upon treatment with RNA-G4 stabilizing ligand carboxyPyridostatin
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| Contributor(s) |
Sauer M, Juranek SA, Benhalevy D, Wang X, Hafner M, Paeschke K |
| Citation(s) |
31160600 |
| Submission date |
Oct 19, 2017 |
| Last update date |
Sep 30, 2019 |
| Contact name |
Markus Sauer |
| E-mail(s) |
markus.sauer@stud-mail.uni-wuerzburg.de
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| Organization name |
University of Würzburg
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| Street address |
Am Hubland, Biozentrum - Lehrstuhl für Biochemie
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| City |
Würzburg |
| ZIP/Postal code |
97074 |
| Country |
Germany |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE105175 |
DHX36 resolves G-rich structures in mRNA untranslated region to allow their translation |
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| Relations |
| BioProject |
PRJNA415032 |
| SRA |
SRP120502 |
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