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Series GSE105432 Query DataSets for GSE105432
Status Public on Apr 30, 2018
Title Whole-genome transcriptomic analysis of the cell fate switch induced by Notch activation in mouse mammary cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Mice expressing the active form of Notch1(N1ICD) in the basal mammary epithelium (through SMAcreERT mice) were induced to express N1ICD at the onset of puberty (P24). This induces a cell fate switch that results in the acquisition of a luminal identity in the targeted cells. To understand the early signals responsible for this reprogramming of committed basal cells, we analysed by RNAseq the transcriptional changes occurring in the targeted cells after 72h of N1ICD expression. N1ICD-expressing cells express a GFP marker that was used to sort them and GFPpos cells were compared to untargeted basal cells in the same animals (GFPneg).
 
Overall design Three replicates from a pool of 2-3 mice each were used for the analysis. GFPpos and GFPneg cells were sorted from the same replicate. A total of 6 samples was sequenced.
 
Contributor(s) Fre S, Rulands S
Citation(s) 29784917
Submission date Oct 20, 2017
Last update date Jul 25, 2021
Contact name Silvia Fre
Organization name Institut Curie
Department Genetics and Developmental Biology
Lab Notch signaling in stem cells and tumors
Street address 26 rue d’Ulm
City Paris
ZIP/Postal code 75248
Country France
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (6)
GSM2825961 Sample 1 GFPneg
GSM2825962 Sample 1 GFPpos
GSM2825963 Sample 2 GFPneg
Relations
BioProject PRJNA415170
SRA SRP120603

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE105432_RAW.tar 1000.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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