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| Status |
Public on Aug 06, 2018 |
| Title |
Jun promotes axon regeneration by binding enhancer sites and dimerizing with Atf3 |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Axon regeneration is a necessary step toward functional recovery after spinal cord injury. The AP-1 transcription factor c-Jun has long been known to play an important role in directing the transcriptional response of Dorsal Root Ganglion (DRG) neurons to peripheral axotomy that results in successful axon regeneration. Here we performed ChIPseq for Jun in mouse DRG neurons after a sciatic nerve crush or sham surgery in order to measure the changes in Jun’s DNA binding in response to peripheral axotomy. We found that the majority of Jun’s injury-responsive changes in DNA binding occur at putative enhancer elements, rather than proximal to transcription start sites. We also used a series of single polypeptide chain tandem transcription factors to test the effects of different Jun-containing dimers on neurite outgrowth in cortical and hippocampal neurons. These experiments demonstrated that dimers composed of Jun and Atf3 promoted neurite outgrowth in rat CNS neurons. Our work provides new insight into the mechanisms underlying Jun’s role in axon regeneration.
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| Overall design |
This study measures two biological conditions: DRG neurons that received a sciatic nerve axotomy (injured), and DRG neurons that received a sham surgery (uninjured). For each condition, three biological replicates were performed. Each biological replicate consists of the pooled sciatic DRGs (L4-L6) of three adult, male, C57Bl/6 mice. For each biological replicate, the eluate of the chromatin immunoprecipitation for c-Jun is provided along with the input control.
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| Contributor(s) |
Danzi MC, Bixby JL, Lemmon VP |
| Citation(s) |
30077771 |
| Submission date |
Oct 20, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Matt C Danzi |
| E-mail(s) |
m.danzi@med.miami.edu
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| Organization name |
University of Miami
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| Street address |
1095 NW 14th Terrace, Lois Pope LIFE Center 4-26
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| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA415185 |
| SRA |
SRP120606 |
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