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| Status |
Public on Oct 21, 2017 |
| Title |
Expression data from adult wild-type male Drosophila melanogaster fed control or high-sugar diets |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Chronic high-sugar feeding (1 M or 34% sucrose) leads to hyperglycemia, obesity, and insulin resistance in adult flies, compared with those fed a control diet (0.15 M or 5% sucrose). We compared two days and four weeks of high-sugar feeding to look at short- and long- term effects on gene expression.
We used Affymetrix Drosophila GeneChip 2.0 microarrays to quantify differential expression between control and high-sugar-fed flies at two time points.
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| Overall design |
Young adult (3-5 day old) Canton-S genotype D. melanogaster males were fed control or high-sugar diets for two days or 4 weeks. Animals were sacrificed and homogenized on ice, RNA extracted, then cDNA labeled and hybridized by Washington University's Genome Technology Access Center to produce .cel files. dChip (2006 version) was used to extract probeset intensites and to normalize using the precise match/mismatch algorithm.
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| Contributor(s) |
Laura Palanker M, Thomas John B |
| Citation(s) |
29084810 |
| Submission date |
Oct 20, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Laura Palanker Musselman |
| E-mail(s) |
palankl@hotmail.com
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| Organization name |
Binghamton University
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| Department |
Biological Sciences
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| Lab |
S3 room 210
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| Street address |
4400 Vestal Parkway East
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| City |
Binghamton |
| State/province |
New York |
| ZIP/Postal code |
13902 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA415192 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE105448_RAW.tar |
25.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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