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Series GSE10626 Query DataSets for GSE10626
Status Public on May 15, 2008
Title MuRF1-dependent regulation of systemic carbohydrate metabolism as revealed from transgenic mouse studies
Organism Mus musculus
Experiment type Expression profiling by array
Summary Under various pathophysiological muscle-wasting conditions like diabetes and starvation, a family of ubiquitin ligases, including MuRF1 (Muscle specific RING-Finger protein 1), are induced to target muscle proteins for degradation via ubiquitination. In an attempt to identify the in vivo targets of MuRF1 we have generated transgenic mouse lines overexpressing MuRF1 in a skeletal muscle specific fashion. MuRF1-TG lines were viable and had normal fertility. Characterization of their skeletal muscles did not reveal evidence for muscle wasting at 10 weeks of age. In this experiment we compared the skeletal muscle transcriptome of transgenic mice with wildtypes.
Keywords: MuRF1 transgene overexpression
 
Overall design Transcriptional profiling was performed on an Affymetrix Mouse Genome 430 2.0 Array chip. In brief, total RNA from quadriceps was prepared (Quiagen kit RNAeasy kit), reverse transcribed with Superscript II (Invitrogen BRL) and labeled and hybridized as described in the Affymetrix manual. Two pairs of TG/WT at 12 weeks of age were compared.
 
Contributor(s) Hirner S, Krohne C, Labeit S, Labeit D
Citation(s) 18468620
Submission date Feb 25, 2008
Last update date Jun 21, 2012
Contact name Stephanie Hirner
E-mail(s) stephanie.hirner@anaes.ma.uni-heidelberg.de
Phone 0049-(0)621-3831626
Organization name institute of anaesthesiology and intensive care
Department Universitätsklinikum Mannheim
Street address Theodor-Kutzer-Ufer1-3
City Mannheim
ZIP/Postal code 68167
Country Germany
 
Platforms (1)
GPL6526 [Mouse430_2_Mm_UG] Affymetrix GeneChip Mouse Genome 430 2.0 Array [Brainarray Version 9]
Samples (4)
GSM267838 MuRF1 transgene_1
GSM267839 MuRF1 transgene_2
GSM267840 WT1
Relations
BioProject PRJNA107691

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Supplementary file Size Download File type/resource
GSE10626_RAW.tar 22.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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