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| Status |
Public on Nov 01, 2018 |
| Title |
Hsa-miR-371a-5p and hsa-miR-518a-3p regulated genes in choriocarcinoma cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the application of transcriptome sequencing for investigating of the hsa-miR-371a-5p and hsa-miR-518a-3p regulated genes. JAR, JEG-3 and BeWo choriocarcinoma cells were transfected with hsa-miR-371a-5p or hsa-miR-518a-3p inhibitors or control inhibitors. Totally, 237, 132 and 277 genes with > 2 folds change and adjusted P < 0.05 were upregulated in JAR, JEG-3 and BeWo cells respectively after hsa-miR-371a-5p knockdown. Meanwhile, 229, 269 and 191 genes were upregulated in JAR, JEG-3 and BeWo cells respectively after hsa-miR-518a-3p knockdown. The top upregulated genes included many oncogenes or oncogenesis associated ones. Enrichment analysis showed hsa-miR-371a-5p and hsa-miR-518a-3p regulated diverse pathways related to tumorigenesis and metastasis. Our results would be helpful for the searching of early molecular biomarkers and therapeutic targets for gestational trophoblastic neoplasia.
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| Overall design |
Examination of functions of 2 different miRNAs in 3 cell types.
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| |
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| Contributor(s) |
Zhao J, Zhang H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 01, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jiuru zhao |
| Organization name |
International Peace Maternity and Child Health Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
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| Department |
Pathology and Bio-Bank
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| Street address |
Hengshan Road 910#
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| City |
Shanghai |
| ZIP/Postal code |
200030 |
| Country |
China |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA416704 |
| SRA |
SRP123296 |
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