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| Status |
Public on Mar 01, 2008 |
| Title |
Divergent genome expression profiles during hepatic ischemia in young and adult mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Experimental Design:
1. The goal of the experiment:
Age-Related Genome Expression Profiles During Hepatic Ischemia
2. Brief description of the experiment:
Hepatic ischemia/reperfusion (I/R) injury is a complication of liver surgery, transplantation and shock and differs with age. In the present study, we sought to determine if the age-dependent response to I/R injury was related to differential gene expression during the ischemic period. Total RNA of young (4-5 weeks) and adult (12-14 months) mice undergoing sham surgery or partial hepatic ischemia for 30, 60, or 90 minutes was analyzed by Affymetrix microarray. Gene expression was filtered based on a change in expression of 1.5-fold relative to respective controls and then analyzed by ANOVA. Significant differences in gene expression were observed between age groups. In young mice, 169 genes were down-regulated, whereas adult mice had 1167 genes down-regulated. Of these, only 28 genes were down-regulated in both young and adult mice. Far fewer genes had increased expression during ischemia. Sixty genes in young mice and 51 genes in adult mice were up-regulated. Of those, none were up-regulated in both young and adult mice. There were no distinct functional patterns of gene regulation in either age group. The data demonstrate significant and widespread changes in hepatic gene expression during the ischemic period that may be important to the age-dependent response to I/R injury.
Keywords: treated vs non treated
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| Overall design |
2 age groups of 12 young and 12 adult mice that underwent either 30, 60 or 90 minutes of partial ischemia (n=3) or sham operation (n=3)
We used microarray to uncover the genomic response during different time points of ischemia
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| Contributor(s) |
Huber N, Eismann T, Shin T, Kuboki S, Galloway E, Schuster R, Wong HR, Lentsch AB |
| Citation(s) |
19206148 |
| Submission date |
Feb 27, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Bhuvaneswari Sakthivel |
| E-mail(s) |
bhuvana.sakthivel@cchmc.org
|
| Phone |
513-636-0146
|
| Fax |
513-636-2056
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| Organization name |
CCHMC
|
| Department |
Biomedical Informatics
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL5759 |
Affymetrix GeneChip Mouse Genome 430 2.0 Array [CDF: Mm430_Mm_REFSEQ_8] |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE10657 |
Hepatic response to ischemia and reperfusion: young and adult mice |
|
| Relations |
| BioProject |
PRJNA108845 |
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