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| Status |
Public on Nov 13, 2017 |
| Title |
HUH VP30 cells with and without interferon α and β treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The purpose is to obtain HUH VP30 samples for mRNA analysis with and with out interferon α and β treatment. Huh VP30 cells have been stably transfected with the Ebola VP30 gene, enabling replication of VP30-deficient Ebola virus. PubMed ID 18212124 and 19211761.
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| Overall design |
Treatments were initiated by replacing culture medium (containing 10% FBS) with fresh medium containing 250 U/ml of human interferon α and 250 U/ml of human interferon β(PBL Biosciences) with a multiplicity of 5 PFU per cell. Mock-treated cells were given normal culture medium only. Mock and treated cells were incubated at 37°C until sample collection time points of 6, 12, and 18 hours. MERSCOV003.0P references the write up of the experimental procedure.
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| Contributor(s) |
Baric R, Sims A, Heller N, Waters KM, Eisfeld AJ, Kawaoka Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 03, 2017 |
| Last update date |
Jan 24, 2018 |
| Contact name |
Natalie Heller |
| E-mail(s) |
natalie.heller@pnnl.gov
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| Organization name |
PNNL
|
| Street address |
902 Battelle Blvd.
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| City |
Richland |
| ZIP/Postal code |
99354 |
| Country |
USA |
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| Platforms (1) |
| GPL13497 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE65575 |
Modeling Host Responses to Understand Severe Human Virus Infections |
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| Relations |
| BioProject |
PRJNA417084 |
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