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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 31, 2020 |
| Title |
Endogenous expression of CD83 in Foxp3+ T cells essentially contributes to differentiation and function of murine Foxp3+ effector regulatory T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Foxp3-positive regulatory T (Treg) cells are crucial players for the maintenance of immune homeostasis and keep immune responses in check. Upon activation, Treg are transferred into an effector state expressing transcripts essential for their suppressive activity, migration and survival. However, how different intrinsic and environmental factors control differentiation is still not completely understood. Here, we present for the first time data suggesting that Treg intrinsic expression of CD83 is essential for Treg differentiation upon activation. Interestingly, mice with Treg intrinsic CD83 deficiency are characterized by a pro-inflammatory phenotype. Furthermore, the loss of CD83 expression by Treg leads to the downregulation of Treg specific differentiation markers and the induction of an inflammatory profile. In addition, Treg specific conditional knockout mice showed aggravated autoimmunity and an impaired resolution of inflammation. Altogether, CD83 expression in Treg cells is an essential factor for the development and function of effector Treg cells upon activation.
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| Overall design |
Whole genome transcript levels were measured in 3h activated versus non-activated (anti-CD3/-CD28) splenic sorted Treg total RNA preparations from 6 mice of two different genotypes (in general three 9-10-week-old mice per genotype)
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| Contributor(s) |
Doebbeler M, Koenig C, Krzyzak L, Seitz C, Butterhof A, Kuhnt C, Kuzcera K, Sandrock L, Zinser E, Knippertz I, Wirtz S, Riegel C, Hoffmann P, Nitschke L, Winkler T, Kopelyanskiy D, Ulas T, Schultze J, Steinkasserer A, Lechmann M |
| Citation(s) |
29875316 |
| Submission date |
Nov 06, 2017 |
| Last update date |
Jun 30, 2020 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
j.schultze@uni-bonn.de
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| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
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| Department |
Genomics and Immunoregulation
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| Street address |
Carl-Troll-Strasse 31
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| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA417322 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE106598_RAW.tar |
143.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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