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Series GSE106674 Query DataSets for GSE106674
Status Public on Nov 09, 2017
Title Transcriptome change caused by lowered intracellular S-adenosylmethionine level in the X63/0 mouse plasma cell line.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We aimed to examine the gene expression changes responding to a depletion of intracellular S-adenosylmethionine (SAM). The mouse plasma cell line X63/0 was treated with the SAM-synthetase inhibitor cycloleucine (cLEU), and the total RNA was isolated and analyzed by RNA-sequencing. As a result, we idntified 27 genes, including the ubiquitous SAM-synthetase MAT2A, whose expssions were up-regulated by two-fold or more.
 
Overall design Total RNA was extracted from the mouse plasma cell line X63/0 before/after a three-hour treatment with 30 mM cycloleucine. This experiment was triplicated, and the resulting six samples were applied to RNA-sequencing.
 
Contributor(s) Matsumoto M, Shima H, Igarashi K
Citation(s) 29262316
Submission date Nov 08, 2017
Last update date May 15, 2019
Contact name Mitsuyo Matsumoto
E-mail(s) m-matsumoto@med.tohoku.ac.jp
Organization name Tohoku university school of medicine
Department Biochemistry
Street address 2-1 Seiryo-machi Aoba-ku
City Sendai
ZIP/Postal code 980-8575
Country Japan
 
Platforms (1)
GPL18635 Ion Torrent Proton (Mus musculus)
Samples (2)
GSM2845123 X63/0_cell_non-treated
GSM2845124 X63/0_cell_cycloleucine-treated
Relations
BioProject PRJNA417590
SRA SRP124606

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE106674_X63_CLEU_Mat2a.txt.gz 2.5 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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