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Series GSE106700 Query DataSets for GSE106700
Status Public on Nov 10, 2017
Title Characterization of transcriptional and epigenetic changes during mouse alternative macrophage polarization [RNA-Seq I]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The molecular basis of signal-dependent transcriptional activation has been extensively studied in macrophage polarization, however our understanding remains limited regarding the molecular determinants of repression. Here we showed that IL-4-activated STAT6 transcription factor is required for the direct transcriptional repression of a large number of genes during mouse alternative macrophage polarization. Repression results in decreased lineage-determining transcription factor, p300 coactivator and RNA polymerase II binding followed by reduced enhancer RNA expression, H3K27 acetylation and chromatin accessibility. In addition, STAT6-repressed enhancers showed extensive overlap with the NF-κB p65 transcription factor cistrome and exhibited decreased responsiveness to lipopolysaccharide after IL-4 stimulus on a subset of genes.
 
Overall design PRJNA416285
 
Contributor(s) Horvath A, Nagy L
Citation(s) 29343442, 30799488
BioProject PRJNA416285
Submission date Nov 08, 2017
Last update date May 15, 2019
Contact name Gergely Nagy
Organization name University of Debrecen
Department Department of Biochemistry and Molecular Biology
Street address Egyetem ter 1.
City Debrecen
ZIP/Postal code 4032
Country Hungary
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (12)
GSM2845594 RNA-Seq analysis of Wt mouse BMDM cells differentiated in the presence of MCSF (ctrl_ctrl_rep1)
GSM2845595 RNA-Seq analysis of Wt mouse BMDM cells differentiated in the presence of MCSF (24hIL4_ctrl_rep1)
GSM2845596 RNA-Seq analysis of Wt mouse BMDM cells differentiated in the presence of MCSF (ctrl_3hLPS_rep1)
This SubSeries is part of SuperSeries:
GSE106706 Characterization of transcriptional and epigenetic changes during mouse alternative macrophage polarization
Relations
SRA SRP123511

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Supplementary file Size Download File type/resource
GSE106700_RAW.tar 5.7 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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