|
| Status |
Public on Nov 10, 2017 |
| Title |
Characterization of transcriptional and epigenetic changes during mouse alternative macrophage polarization [ATAC-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The molecular basis of signal-dependent transcriptional activation has been extensively studied in macrophage polarization, however our understanding remains limited regarding the molecular determinants of repression. Here we showed that IL-4-activated STAT6 transcription factor is required for the direct transcriptional repression of a large number of genes during mouse alternative macrophage polarization. Repression results in decreased lineage-determining transcription factor, p300 coactivator and RNA polymerase II binding followed by reduced enhancer RNA expression, H3K27 acetylation and chromatin accessibility. In addition, STAT6-repressed enhancers showed extensive overlap with the NF-κB p65 transcription factor cistrome and exhibited decreased responsiveness to lipopolysaccharide after IL-4 stimulus on a subset of genes.
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| |
|
| Overall design |
PRJNA416285
|
| |
|
| Contributor(s) |
Horvath A, Nagy L |
| Citation(s) |
29343442, 30799488 |
| BioProject |
PRJNA416285 |
| Submission date |
Nov 08, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Gergely Nagy |
| Organization name |
University of Debrecen
|
| Department |
Department of Biochemistry and Molecular Biology
|
| Street address |
Egyetem ter 1.
|
| City |
Debrecen |
| ZIP/Postal code |
4032 |
| Country |
Hungary |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (4)
|
| GSM2845670 |
ATAC-Seq analysis of Wt mouse BMDM cells differentiated in the presence of MCSF (ctrl, rep1) |
| GSM2845671 |
ATAC-Seq analysis of Wt mouse BMDM cells differentiated in the presence of MCSF (ctrl, rep2) |
| GSM2845672 |
ATAC-Seq analysis of Wt mouse BMDM cells differentiated in the presence of MCSF (1hIL4, rep1) |
| GSM2845673 |
ATAC-Seq analysis of Wt mouse BMDM cells differentiated in the presence of MCSF (1hIL4, rep2) |
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| This SubSeries is part of SuperSeries: |
| GSE106706 |
Characterization of transcriptional and epigenetic changes during mouse alternative macrophage polarization |
|
| Relations |
| SRA |
SRP123511 |
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