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| Status |
Public on May 10, 2018 |
| Title |
Identification of SoxC-regulated genes during neurogenesis in the developing spinal cord [chicken] |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by array
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| Summary |
The HMG-domain containing SoxC transcription factors Sox4 and Sox11 are expressed in the vertebrate central nervous system in neuronal precursors and neuroblasts. They are required during early stages of neurogenesis.
Here we perform micorarray analysis to identify genes that are downstream of these SoxC proteins during spinal cord neurogenesis in chicken. The identified genes represent potential direct target genes of Sox4 and Sox11.
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| Overall design |
The neural tube of chicken embryos were electroporated at HH9 stage with shRNA expression plasmids . Electroporated cells were obtained by FACS from neural tubes at HH11 and used to prepare total RNA. Overall gene expression patterns were compared between control transfected cells and cells transfected with Sox11- and Sox4-shRNAs using Affymetrix microarrays.
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| Contributor(s) |
Fröb F, Thalhammer J, Sock E, Wegner M |
| Citation(s) |
29749639 |
| Submission date |
Nov 12, 2017 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Michael Wegner |
| Organization name |
Friedrich-Alexander Universität Erlangen-Nürnberg
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| Department |
Institut of Biochemistry
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| Lab |
Michael Wegner
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| Street address |
Fahrstr. 17
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| City |
Erlangen |
| State/province |
Bavaria |
| ZIP/Postal code |
91054 |
| Country |
Germany |
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| Platforms (1) |
| GPL3213 |
[Chicken] Affymetrix Chicken Genome Array |
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| Samples (2) |
| GSM2850273 |
chicken embryo HH11, neural tube electroporated with pSuper-neo-GFP |
| GSM2850274 |
chicken embryo HH11, neural tube electroporated with pSuper-neo-GFP-Sox4-shRNA + pSuper-neo-GFP-Sox11-shRNA |
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| This SubSeries is part of SuperSeries: |
| GSE106788 |
Identification of SoxC-regulated genes during neurogenesis in the developing spinal cord |
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| Relations |
| BioProject |
PRJNA418041 |
