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Status |
Public on Dec 12, 2017 |
Title |
Analysis of RNA polymerase stalling index in wild type and the rel- strain of Synechococcus elongatus PCC7942 - chromatin immunoprecipitation followed by sequencing during exposure to darkness (cells grown in light/dark conditions) |
Organism |
Synechococcus elongatus PCC 7942 = FACHB-805 |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
We investigated the role of ppGpp in Synechococcus elongatus PCC7942 in light/dark conditions. We performed ChIP-seq experiments using wild type and the rel- strain at dusk, during exposure to 12 h of darkness and at dawn. We show that the RNA polymerase pausing index is globally lower in the rel- strain.
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Overall design |
Enriched mRNA was prepared from synchronized S. elongatus cells at indicated time points using the Illumina TruSeq Stranded mRNA Sample Prep Kit and Illumina HiSeq technology.
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Contributor(s) |
Puszynska AM, O'Shea EK |
Citation(s) |
29241543 |
Submission date |
Nov 13, 2017 |
Last update date |
Jul 20, 2022 |
Contact name |
Anna M Puszynska |
E-mail(s) |
apuszyns@fas.harvard.edu
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Organization name |
Harvard University
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Department |
Molecular and Cellular Biology
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Lab |
O'Shea
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Street address |
52 Oxford St, Room 445
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City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02138 |
Country |
USA |
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Platforms (1) |
GPL16957 |
Illumina HiSeq 2000 (Synechococcus elongatus PCC 7942) |
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Samples (12)
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This SubSeries is part of SuperSeries: |
GSE103606 |
ppGpp controls global gene expression in light and in darkness in S. elongatus |
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Relations |
BioProject |
PRJNA418146 |
SRA |
SRP124853 |