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Series GSE106824 Query DataSets for GSE106824
Status Public on Dec 12, 2017
Title Analysis of RNA polymerase stalling index in wild type and the rel- strain of Synechococcus elongatus PCC7942 - chromatin immunoprecipitation followed by sequencing during exposure to darkness (cells grown in light/dark conditions)
Organism Synechococcus elongatus PCC 7942 = FACHB-805
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We investigated the role of ppGpp in Synechococcus elongatus PCC7942 in light/dark conditions. We performed ChIP-seq experiments using wild type and the rel- strain at dusk, during exposure to 12 h of darkness and at dawn. We show that the RNA polymerase pausing index is globally lower in the rel- strain.
 
Overall design Enriched mRNA was prepared from synchronized S. elongatus cells at indicated time points using the Illumina TruSeq Stranded mRNA Sample Prep Kit and Illumina HiSeq technology.
 
Contributor(s) Puszynska AM, O'Shea EK
Citation(s) 29241543
Submission date Nov 13, 2017
Last update date Jul 20, 2022
Contact name Anna M Puszynska
E-mail(s) apuszyns@fas.harvard.edu
Organization name Harvard University
Department Molecular and Cellular Biology
Lab O'Shea
Street address 52 Oxford St, Room 445
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platforms (1)
GPL16957 Illumina HiSeq 2000 (Synechococcus elongatus PCC 7942)
Samples (12)
GSM2854964 wild type, dusk
GSM2854965 wild type, 4h dark
GSM2854966 wild type, 8h dark
This SubSeries is part of SuperSeries:
GSE103606 ppGpp controls global gene expression in light and in darkness in S. elongatus
Relations
BioProject PRJNA418146
SRA SRP124853

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE106824_Stalling_index.xlsx 150.0 Kb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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