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| Status |
Public on Nov 18, 2017 |
| Title |
Gene expression profiling of mouse macrophages following long noncoding RNA Malat1 knockdown |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We applied next-generation sequencing to investigate the gene expression profiles in mouse bone-marrow derived macrophages with or without long noncoding RNA-Malat1 knockdown. We identified a number of differentially regulated genes in cells with Malat1 knockdown.
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| Overall design |
Mouse bone-marrow derived macrophages were transfected with Antisense LNA™ GapmeRs against Malat1 or Negative Control oligos (Exiqon). 48h after transfection, total RNA was isolated and subjected to high-throughput sequencin (RNA-seq), using Illumina GAIIx. Gene expression profiles were compared between two groups.
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| |
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| Contributor(s) |
Liu G, Cui H |
| Citation(s) |
30676324 |
| Submission date |
Nov 15, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Huachun Cui |
| E-mail(s) |
hcui@uab.edu
|
| Organization name |
University of Alabama at Birmingham
|
| Street address |
BMR2-274 901 19TH ST S
|
| City |
Birmingham |
| State/province |
Alabama |
| ZIP/Postal code |
35294 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA418453 |
| SRA |
SRP125035 |
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